Standard

Beklager, dette produktet er ikke lenger tilgjengelig

prNS-EN ISO 17174:2023

Tilbaketrukket

Merknad: Denne standarden har en ny utgave: NS-EN ISO 17174:2024

Forhåndsvisning
For bestilling og priser av dette produktet ta kontakt med salg@standard.no

Omfang

This document describes a procedure for the identification of single fish and fish fillets to thelevel of genus or species.The identification of fish species is carried out by PCR amplification of either a segment of themitochondrial cytochrome b gene (cytb) [1] or the cytochrome c oxidase I gene (cox1, syn COI)[2], [3] or both, followed by sequencing of the PCR products and subsequent sequencecomparison with entries in databases [4], [5]. The methodology allows the identification of a largenumber of commercially important fish species.The decision whether the cytb or cox1 gene segment or both are used for fish identificationdepends on the declared fish species, the applicability of the PCR method for the fish speciesand the availability of comparative sequences in the public databases.This method has been successfully validated on raw fish fillets, however, laboratory experienceis available that it can also be applied to processed, e.g. cold smoked, hot smoked, salted,frozen, cooked, fried, deep friedsamples.This document is usually unsuitable for the analysis of highly processed foods, e.g. tins of fish,with highly degraded DNA where the fragment lengths are not sufficient for amplification of thetargets. Furthermore, it is not applicable for complex fish products containing mixtures of two ormore fish species.

Dokumentinformasjon

  • Standard fra SN
  • Utgave: 2023-12
  • Versjon: 1
  • Varetype: NAT
  • ICS 67.120.30
  • National Committee CEN/TC 460

Produktrelasjon

Produkt livssyklus